mix up 1 teasp of liquid hand soap & 1 qt spray spray in the hole.............do it again.......................spray the whole tree.....................spray again and keep them moving..............you could use spectracide tree spray to close the hole or some tar. 2 parts animals are termites.............3 part animals are ants....................if they are termites this will not work......you did not mention what kind of tree if this is termites you need something much more strong that what I mentioned.